Coding

Part:BBa_K5374014:Design

Designed by: Langning Xu   Group: iGEM24_TestDaily-I-Beijing   (2024-09-17)


AGR-EGFP (Agrin Domain). A multi-domain proteoglycan that binds collagen, fused with EGFP to determi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
    Illegal PstI site found at 826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 1309
    Illegal PstI site found at 352
    Illegal PstI site found at 826
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
    Illegal PstI site found at 826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
    Illegal PstI site found at 826
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1952


Design Notes

Optimized for EGFP fusion to maintain both collagen-binding capacity and fluorescent reporting for activity assays.


Source

Derived from agrin gene in mammals, with EGFP from BBa_K1911005.

References